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L Wis4(1-300) segment followed by the central region of Win1 (chimeras 4-1-1 and 4-1-4 in Figure 4C). Based on the Wis4 in1 interaction analysis described earlier, these chimeras should allow homophilic interactions. Their ability to induce activation of the Spc1 MAPK cascade was assayed in the absence of the endogenous Wis4 and Win1 MAPKKKs. We found that both 4-1-1 and 4-1-4 chimeras induce str
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